【摘要】 目的 :构建乙酰肝素酶信号密码的反义基因真核表达载体 ,探索恶性肿瘤基因治疗的新途径。方法 :以质粒 pc DNA3.1为载体 ,将乙酰肝素酶信号密码的 DNA片段反向插入其多克隆位点得到反义乙酰肝素酶信号密码真核表达载体 ( pc DNA3.1 - as Hpa)。结果 :经过 PCR扩增鉴定、电泳证实获得的重组基因的大小和插入方向正确。结论 :成功的构建了乙酰肝素酶信号密码的反义基因真核表达载体 ,可以进一步用于反义基因表达的实验研究更多还原

【Abstract】 Objective:To construct of human antisense heparanase signal code gene (pcDNA3.1-asHpa) and explore a new method in tumor therapy. Methods: Human heparanase signal code DNA were inserted into the expression plasmid pcDNA3.1 site in an antisense orientation to produce pcDNA3.1-asHpa. Results: The PCR and electrophoresis were carried out,and the antisense expression vector was verified. Conclusion: Human antisense heparanase signal code gene pcDNA3.1 vector has been constructed and could be used for related study of antisense gene expression.更多还原