【摘要】 目的　探讨Wistar大鼠诱导实验性变态反应脑脊髓炎 (EAE)动物模型 ,检测EAE中起关键趋化作用的巨噬细胞趋化因子 - 1(MCP- 1)的表达。方法　采用免疫诱导方法制备EAE模型并苏木素 伊红 (HE)染色 ,同时用原位杂交法检测EAE大鼠MCP -1的表达。结果　免疫后 12～ 17d ,发病鼠出现EAE临床症状 ,HE染色 ,光镜下可见血管周围炎性浸润 ,MCP- 1的表达明显增加。结论　Wistar大鼠成功的诱导实验性变态反应脑脊髓炎模型是可信、可用的。更多还原

【Abstract】 Objective Establishing experimental autoimmune encephalomyelitis (EAE) model in insensitive Wistar rats from Shanxi Medical University to observe expression of MCP-1 mRNA.Methods With complete antigen and PT to immunize Wistar rats,and inoculate for 12～17 days;their brains and spinal cords were removed for HE staining and in situ hybridization (ISH).Results The animal got the clinical symptom of EAE;the characteristic light microscopical findings:perivascular inflammatory infiltration,and increased MCP-1 expression.Conclusion The EAE model of Wistar rats made up with the method is stable and reliable.更多还原