【摘要】 目的探讨乳腺肌上皮细胞标志物p63、-αSMA和Calponin在乳腺良恶性疾病中的表达及意义。方法收集乳腺癌标本67例及其癌旁组织。将其分为5组:癌旁正常乳腺组、乳腺增生组、乳腺导管不典型增生组、原位癌组和浸润癌组。应用免疫组织化学S-P法检测p63、-αSMA和Calponin在乳腺癌中的表达。结果在乳腺正常组、增生组、不典型增生组中,几乎所有的肌上皮细胞表达p63、-αSMA和Calponin,而所有的腺上皮细胞3种抗体均为阴性;在原位癌组中3种抗体均表现为:癌巢外围可见p63、-αSMA或Calponin阳性的肌上皮细胞完整或部分包绕;在浸润癌组所有的癌巢外缘均未见p63、α-SMA或Calponin阳性的肌上皮细胞。结论与-αSMA、Calponin相比,p63显示乳腺肌上皮细胞具有相对较高的敏感性和特异性,可作为肌上皮细胞的又一有效的标志物。更多还原

【Abstract】 Objective To investigate the expression and significance of p63,α-SMA and Calponin proteins in benign and malignant lesions of breast.Methods Sixty-seven cases of breast carcinoma,containing normal breast tissue were divided into 5 groups,the expression of p63,α-SMA and Calponin were detected by immunohistochemistry in each group.Results In normal breast tissue group,usual hyperplasia and atypical ductal hyperiplasia groups,p63,α-SMA and Calponin were positive in almost all of the myoepithelial cells,and the above antibodies were negative in all the breast epithelial cells.In the carcinoma in situ group,p63,αSMA and Calponin showed:some cancer cell nests showing continuous positive myoepithelial cells(MECs) in their circumference,others showing discontinuous positive MECs or entirely no positive MECs.There were no p63 or α-SMA or Calponin positive myoepithelial cells in invasive carcinoma group.Conclusion Although antibodies to p63 offer excellent sensitivity and increased specificity for myoepithelial cell detection relative to antibodies to Calponin and α-SMA,they have the following diagnostic limitations:they react with a small subset of breast carcinoma cells.更多还原