【摘要】 目的通过分析再生障碍性贫血(再障)患者外周血T细胞亚群及Th1和Th2细胞,进一步探讨再障的免疫发病机制。方法①流式细胞术检测再障患者外周血T细胞亚群和T细胞HLADR抗原的表达;②用流式细胞术检测经体外刺激后再障患者外周血单个核细胞(PBMNC)中Th1、Th2细胞。结果①再障患者CD3+CD4+细胞、CD3+CD8+细胞、CD8+HLADR+细胞百分率及CD4+/CD8+比值分别为(35.05±9.47)%,(36.29±6.81)%,(18.36±2.07)%,1.0±0.33;对照组为(52.84±4.03)%,(24.69±3.90)%,(11.13±1.14)%,2.14±0.24;再障患者CD3+CD4+细胞和CD4+/CD8+比值显著低于对照组(P<0.01);CD3+CD8+细胞、CD8+HLADR+细胞显著高于对照组(P<0.01);而CD3+细胞与对照组相比差异无显著性(P>0.05)。②再障患者外周血中Th1细胞百分率和Th1/Th2比值分别为(20.62±2.98)%和14.63±4.81,均显著高于对照组(11.13±1.48)%和9.33±2.82(P<0.01);而Th2细胞百分率与对照组相比差异无显著性(P>0.05)。③Th1细胞与CD3+CD8+细胞呈正相关(r为0.678,P<0.01),与网织红细胞相对值、中性粒细胞绝对值(ANC)呈负相关(r分别为-0.590和-0.503,P值均<0.05)。结论再障患者的T淋巴细胞亚群失衡,Th1细胞增多可能是导致再障造血衰竭的重要环节。更多还原

【Abstract】 Objective To determine the cellular immunological abnormalities in aplastic anemia(AA). Methods ①The quantity of the subsets of T lymphocyte and CD8~+HLA-DR~+ cells in peripheral blood of AA patients at diagnosis were detected respectively by FACS.②Th1 and Th2 cells in peripheral blood mononuclear cells(PBMNC) of AA patients were counted by FACS. Results ①In aplastic anemia patients,the percentage of CD3~+CD4~+ cells,CD3~+CD8~+cells,CD8~+HLA-DR~+cells and the ratio of CD4~+/CD8~+ were (35.05±9.47)%,(36.29±6.81)%,(18.36±2.07)% and 1.0±0.33 respectively.In normal controls,they were (52.84±4.03)%,(24.69±3.90)%,(11.13±1.14)% and 2.14±0.24 respectively.The percentage of CD3~+CD4~+ cells and the ratio of CD4~+/CD8~+ of AA patients were significantly lower than that of controls;the percentage of CD3~+CD8~+ cells and CD8~+HLA-DR~+ cells were significantly higher than that of controls;while the percentage of CD3~+cells had no difference between AA patients and normal controls.②The percentage of Th1 cells and the ratio of Th1/Th2 of AA patients were significantly higher than that of controls,the percentage of Th2 cells had no difference between AA patients and normal controls.③The percentage of Th1 cells in AA patients were positively correlated with their CD3~+CD8~+cells and negatively correlated with their RC and ANC. Conclusion Disbalance of T lymphocyte subsets and increases in quantity of Th1 cells might be important for the development of bone marrow failure in aplastic anemia.更多还原