【摘要】 目的探讨内毒素脂多糖(LPS)对胃粘膜上皮细胞RGM-1的作用及其机制。方法细胞培养液中加入不同浓度的吲哚美辛,以2,4-二硝基苯肼法测定培养上清液中乳酸脱氢酶(LDH)的含量来反映细胞受损情况。采用Westernblot印迹法检测细胞中COX-1、COX-2的表达情况并用放免法测定培养上清液中前列腺素E2(PGE2)含量的变化。结果低浓度的吲哚美辛即可引起RGM-1细胞受损并且呈量效关系,LPS可以明显减轻这一作用(P<0.05);Westernblot分析发现LPS可以诱导COX-2的表达;放免结果表明LPS作用后培养上清液中PGE2的含量增加(P<0.05)。结论LPS对RGM-1细胞株有保护作用,这一作用是通过刺激COX-2的表达实现的。更多还原

【Abstract】 Objective To explore the effect and mechanism of lipopolysaccharide （LPS） on rat gastric mucosal cells line （RGM-1）. Methods Injury of rat gastric mucosal cell line was examined by lactate dehydrogenase leakage. Western blot asssy was used to study the changes of COX-1 and COX-2. The level of PGE₂ was determined by radioimmunoassay. Results The result of LDH leakage showed that indomethacin could induce the injury of RGM-1 in a dose dependent manner and LPS could lessen it significantly （P<0.05）. Western blot assay showed that LPS could induce the expression of COX-2, and radioimmunossay indicated that the quatility of PGE₂ increased significantly （P<0.05）. Conclusion LPS has cytoprotective effect on RGM-1 via involving overexpression of COX-2.更多还原