【摘要】 目的检测肺癌患者支气管灌洗液及全DNA中p16基因甲基化,并探讨其在肺癌早期诊断中的作用。方法利用半巢式甲基化特异性PCR技术,检测26例肺癌患者支气管灌洗液及相应全血中DNA p16基因的异常甲基化状态。结果34.6%(9/26)肺癌组织出现p16基因甲基化,出现甲基化组织对应的血液标本中88.9%(8/9)出现p16基因甲基化。支气管灌洗液中p16基因的甲基化状况与全血中是否出现p16基因甲基化关系密切(P<0.01)。结论肺癌患者支气管灌洗液及血液标本中p16基因甲基化的检测可能有助于肺癌的早期诊断。更多还原

【Abstract】 Objective To detect methylation in p16 gene in whole blood and bronchia rinse of lung cancer,and to explore its significance for early diagnosis in lung cancer.Methods Using a modified semi-nested methylation-specific PCR(MSP),the status of methylation of the p16 was investigated in bronchia rinse and whole blood DNA from 26 1ung cancer patients.Results Methylated p16 gene was found in 34.6%(9/26) of lung cancer and in 88.9%(8/9) whole blood samples,methylated p16 gene promoter in whole blood strongly correlated with tissue of lung cancer.Conclusion The measurement of methylated p16 gene may be helpful to early diagnosis for lung cancer.更多还原