【摘要】 应用RAPD技术对Boer山羊与山西地方山羊品种的遗传多态性进行了研究,利用基因频率进行了聚类分析,结果表明:1)四个品种的RAPD标记具有丰富的多态性,多态性带占总带数的63.81%,单个引物获得RAPD带数在3～10之间,各片段分子量大小在200～3252bp之间,所得多态性位点频率的变化范围在50%～81.8%。2)聚类分析表明:黎城大青羊与阳城白山羊首先聚为一类,具有较近的亲缘关系,吕梁黑山羊具有独特的遗传特性。山西主要地方山羊聚为一类,与国外品种波尔山羊亲缘关系较远。3)根据对4个品种遗传差异的分析,认为波尔山羊与吕梁黑山羊进行杂交组合,可望获得较高的杂种优势。更多还原

【Abstract】 Genetic polymorphism and cluster analysis on Boer and Shanxi native goats (Yangcheng White、Licheng Daqing、Luliang Black) werestudied using randomly amplified polymorphic DNA (RAPD) technique, the results of this study indicatedas follows:1)The DNA of 4 breeds provideda great number of molecular markers with high polymorphism. One hundred and five loci were generated, 67 loci (63.81%) of which were highly polymorphic and 38 loci were commonfor all samples. Every single primer generated three to ten RAPD bands and all amplified DNA fragments in length were between 200~3252 bp withpolymorphic frequency from 50%~81.8%.2)Cluster analysis showed that the Shanxi native goats had a larger genetic distance with Boer, Licheng Daqing and Yangcheng White from alike region had a smaller distance. Luliang Black had its special genetic characteristics.3 ) According to the theory of heterosis and analysis on the genetic diversity of 4 breeds, the higher heterosis may be gained in cross combination betweenBoer and Luliang Black.更多还原