【摘要】 用农杆菌介导法对芥菜型油菜进行了转化,并对影响根癌农杆菌转化效率的因素进行了研究,建立了以油菜子叶柄为外植体的转化体系.用携带蝎毒素(BmKITs)和几丁质酶(chi)双价基因的农杆菌转化油菜,共获得了抗卡那霉素的再生苗153株,移栽成活21株.对成活植株进行了PCR分子检测,证实有外源基因的整合.转基因植株形态正常,开花、结实.更多还原

【Abstract】 Factors influencing Brassica juncea transformation mediated by Agrobacterium tumfaciens were studied.The results showed that sterilization of oil rape seeds using 0.1 % HgCl₂ was better for the growth of sterile seedling.When the petioles were infected by A.tumfaciens for 10 min,the efficiency of transformation was the highest and the callus had the least browning.An efficient and stable B.juncea transgenic system was established.The binary anti-insect genes were transferred into B.juncea via A.tumfaciens-mediated transformation.153 Km-resistant shoots were produced,and 21 regenerated plants survived.The result by PCR showed that the insect-resistant genes had been integrated into the genome of transgenic plants.All the transformants were nornal in morphology.更多还原