【摘要】 目的观察LHRH-PE40对宫颈癌Hela细胞系凋亡及增殖的影响,为其临床应用提供理论依据。方法采用人宫颈癌Hela细胞株进行培养,在培养液中分别加入不同浓度的LHRH-PE40,作用48h后,观察Hela细胞形态的变化,并通过结晶紫染色法检测宫颈癌Hela细胞株对LHRH-PE40的感受性;用流式细胞仪及免疫组化法检测LHRH-PE40对Hela细胞周期、Ki-67及Bcl-2的影响。结果LHRH-PE40对Hela细胞活性的抑制作用呈剂量依赖性,通过量效曲线可以看出,LHRH-PE40的最小有效剂量为0·625μg/ml;半数致死剂量为0·969μg/ml。在1·0μg/ml浓度下,癌细胞中的Ki-67阳性细胞(用药前80%,用药后42%)及Bcl-2阳性细胞(用药前66%,用药后43%)比率均下降,凋亡细胞比率增加(由0增加到3·7%)。在1·0μg/ml浓度下,细胞的G0/G1期细胞比率增加15·21%;S期细胞变化不大,而G2/M期减少38·28%。结论LHRH-PE40可以有效地抑制宫颈癌细胞的增殖,诱发肿瘤细胞凋亡,是一种很有应用前景的抗肿瘤药物。更多还原

【Abstract】 Objective To observe the influence of LHRH-PE40 on the apoptosis and proliferation of cer vical carcinoma Hela cell line. Methods Culture the Hela cells of human cervical carcinoma in vitro,add LHRH-PE40 at di fferent concentrations into the medium,incubate for 48 h and observe the morphol ogy of the cells.Detect the sensitivity of Hela cells to LHRH-PE40 by crystal v iolet staining,the cell cycle by flow cytometry,and the Ki-67 and Bcl-2 by imm unohistochemical method. Results LHRH-PE40 showed a dose-dependent inhibitory effect on the activity of Hela ce lls.The minimum effective and medium lethal dose of LHRH-PE40 were 0.625 and 0 .969 μg/ml respectively.In the presence of 1.0 μg/ml LHRH-PE40,the percenta ges of Ki-67 positive cells and Bcl-2 positive cells decreased from 80% and 66 % to 42% and 43% respectively.However,the percentage of apoptotic cells increase d from 0% to 3.7%.The percentage of cells at G₀/G₁ phases increased by 15. 21%,and that at G₂/M phase decreased by 38.28%.The percentage of cells at S p hase showed no significant change. Conclusion LHRH-PE40 may effectively inhibit the proliferation of cervical carcinoma cells and induce the apoptosis of tumor cells.It might be a candidate of anti-tumor drug.更多还原