【摘要】 构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒,并在大肠肝菌中表达。用PCR扩增PTD-BDNF融合基因,经DNA测序无误后插入表达质粒pJW2构建质粒pJPB,转化大肠杆菌并进行PTD-BDNF蛋白的诱导表达和纯化。结果DNA序列分析表明构建含有PTD-BDTF融合基因的质粒与设计相同,PTD-BDNF融合蛋白在大肠杆菌中获得表达并进一步纯化。更多还原

【Abstract】 To construct a recombinant plasmid containing protein transduction domain(PTD) and brain derived neurotrophic factor(BDNF) fusion gene and express in E.coli were studied. PTD-BDNF fusion gene was amplified by PCR and inserted into plasmid pJW2 to get the recombinant plasmid pJPB,which was transformed and expressed in E.coli. PTD-BDNF fusion protein was purified by Sephacry S-100.The PTD-BDNF fusion gene sequencing showed the same sequence as design.The fusion protein was successfully expressed in E.coli and purified.更多还原