【摘要】 比较利用改进的异硫氰酸胍一步法、异硫氰酸胍高盐法、CTAB法和Thomas’RNA提取法等4种方法提取三七根茎2个部位总RNA的可行性。结果表明,改进的异硫氰酸胍一步法和异硫氰酸胍高盐法能有效地抑制酚类物质、多糖及皂苷等次级代谢产物对总RNA的影响,可从三七根茎中获得质量高、完整性好的总RNA。RT-PCR分析显示提取的总RNA具有反转录活性。这2种方法具有快速、简单、有效的特点。更多还原

【Abstract】 The modificated single-step guanidinium isothiocyanate, guanidinium isothiocyanate with high concentration of salt, CTAB extraction method and Thomas’ RNA extraction method were applied to isolate total RNA from root and stem of Panax pseudoginseng var.notoginseng that is rich in polyphenolics, polysaccharides and secondary metabolites such as saponins. The results showed that modificated single-step guanidinium isothiocyanate and guanidinium isothiocyanate with high concentration of salt are more effective. The integrality and high purity of extracted total RNA can meet the demand of molecular biology experiment such as reverse-transcritation by RT-PCR.更多还原