【摘要】 应用超薄切片和免疫金标记电镜技术,结合体视学分析研究了受蚕豆萎焉病毒2(BBWV 2) 中国分离物B935侵染的豌豆(Pisum sativum)叶细胞中线粒体的异常变化。结果表明,感病细胞线粒体增生并聚集于细胞质的膜增生区周围,体积增大,形状畸变,一些线粒体内含有类结晶包涵体。病叶细胞与健康对照之间线粒体的体积密度(VV)、表面积密度(SV)、数密度(NV)等参数存在显著差异(P<0.01),而形状因子(PE)、周长指数(CI)、比表面积(RSV)等参数随不同病变阶段而有变化。在线粒体周围及线粒体之间的网格结构可被BBWV 2金标记抗体特异性标记．推断为正在组装的病毒粒子。子代病毒形成结晶体和管状体,有高密度的免疫金颗粒标记。上述研究结果提示BBWV 2 引起的细胞线粒体异常变化与病毒复制组装有关,聚集线粒体的外膜粘连面可能是病毒粒子组装部位,一些线粒体内的类结晶包涵体可能代表了某种蛋白质异常积累。更多还原

【Abstract】 The alteration of mitochondrion in Pisum sativum leaf cells infected with B935, isolates of BBWV 2, were investigated by electron microscopy, immunogold-labeling technique and stereologic quantity. The results showed that mitochondria were hyperplastic and aggregated nearby the membranous proliferation in cytoplasm. Many mitochondria were enlarged and distorted. There are some square paracrystalline inclusions within abnormal mitochondria. The difference between infected and healthy leaf cells at mitochondrion was observed to reveal significances in volume density (VV), surface density (SV), numerical density (NV)(P<0.01), but it was varied in form factor (PE), circumference index (CI), ratio of surface to volume (Rsv). Some immunogold particles were localized to the crystal substance between the mitochondria. A high density of immunogold particles were localized to the crystalline aggregates and tubules composed of virus particles. This indicated that hyperplasia and aggregation of mitochondrion related to the assembly of virion in infection cells. BBWV 2 particles were assembled at the position of adherent mitochondria. The paracrystalline inclusions within mitochondria could represent the accumulation of a certain protein.更多还原