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柑橘体细胞杂种有性后代的创造及杂种鉴定
CREATION AND HYBRID IDENTIFICATION OF THE SEXUAL PROGENIES USING CITRUS SOMATIC HYBRID AS POLLEN PARENT
【摘要】 以异源四倍体柑橘体细胞杂种[哈姆林甜橙(Citrus sinensis Osbeck)+粗柠檬(C.jambhiri Luss)]为父本与二倍体单胚类型宜本杂4号[华农本地早橘(C.reticulata Blanco)×宜昌橙(C.ichangen- sis Swingle)]杂交,采用胚抢救技术获得了110株有性后代植株,通过染色体计数及倍性分析仪鉴定,其中93株植株为3倍体,余下的17林植株为2倍体。RAPD分析表明:获得的有性后代植株均为杂种。
【Abstract】 110 plantlets were obtained from the cross between citrus allotetraploid somatic hy- brid [Hamlin sweet orange (Citrus sinensis Osback)+Rough Lemon(C.jambhiri Luss)] (as the pollen parent) and mono-embryonic diploid type Iben No.4 [Huanongbendizao tangerine(C. reticu- lata Blanco)×Ichang papeda(C.ichangensis Swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. RAPD analyses showed that all sexual progenies were hybrid.
【Key words】 Citrus somatic hybrid; Allotetraploid; Sexual progeny; Ploidy; Hybrid;
- 【文献出处】 实验生物学报 ,Acta Biologiae Experimentalis Sinica , 编辑部邮箱 ,2005年03期
- 【分类号】S666.2;
- 【被引频次】23
- 【下载频次】355