【摘要】 利用幼苗下胚轴建立了高羊茅(FestucaarundinaceaSchreb.)植株再生体系。当高羊茅种子以次氯酸钠为主要消毒剂时,其最适的浓度和时间分别为次氯酸钠50%(V/V)处理20min,种子发芽率为80%,污染率为0%。研究发现用高羊茅下胚轴诱导愈伤组织时,下胚轴的不同的切法对愈伤组织诱导有影响,从一端切的下胚轴诱导愈伤率74.7%高于从两端切55.8%。以下胚轴为外植体,2,4D的浓度为9mg·L1时,愈伤组织诱导率最高。高羊茅愈伤组织的分化再生率可达80.7%。更多还原

【Abstract】 The regeneration system of the lower plumular axes from tall fescue young seedling has been established in this study. In order to establish a tissue culture system for tall Fescue, different ways of seeds treatment were done to investigate their effects on the germination and pollution rates of seeds, and the results showed that the optimum concentration of sodium hypochlorite was 50%,and the optimal time for treatment was 20 min.The lower plumular of tall fescue axes from two species sliced with two methods were used to induce callus, and the results showed callus induction rates sliced from one end increased 18.9% than that from two ends before plating. The lower plumular axes of tall fescue seedling were inoculated on the MS mediums with different 2,4-D concentration to induce calli, and the results indicated that 9 mg·L -1 2,4-D was the optimum concentration on calli induction, and the regenation rate of calli reached 80.7%.更多还原