【摘要】 目前核酸检测技术在水产养殖疾病诊断中已广泛应用。现就国内外研究发展动向,对分子杂交技术、PCR技术、PCR分子杂交联用技术、PCR免疫学联用技术、16SrRNA技术和限制性酶切技术等方法进行综述,详细阐明了各个技术的原理、特点及应用。病原体遗传背景的研究状况为决定是否应用这些方法的关键。更多还原

【Abstract】 Nucleic acid detection technology has been applied extensively to diagnose of aquatic animal pathogens with the development of molecular biology. This paper introduces the molecular hybridization, polymerase chain reaction(PCR), PCR-molecular hybridization, PCR-immunology, 16S rRNA gene analysis and restriction enzyme digestion technologies, detailedly clarifies their mechanism, characteristics and application in aquaculture. Genetic backgrounds of aquatic animal pathogens play important roles in the application of nucleic acid detection technology in aquaculture.更多还原