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矮化早熟高粱突变体的RAPD分析
RAPD Analysis of Dwarfing and Early Ripening Kaoliang Mutant
【摘要】 以随机扩增多态DNA(RandomAmplifiedPolymorphicDNA,RAPD)标记方法对航天搭载得到的矮化早熟高粱突变体进行遗传特性分析,利用适合玉米的引物对高粱进行引物筛选,从31种随机引物中共扩增出4条多态性片段,反映出突变体的遗传差异性。
【Abstract】 By the marking method of RAPD we analysed the dwarfing and early ripening kaoliang mutant which got from space shuttle. After selected the compatible kaoliang primer from plentiful corn primers,we gained four polymorphic fragments from thirty one random primers. This reflected the genetic difference of the mutant.
【关键词】 矮化早熟;
突变体;
RAPD分析;
遗传差异;
【Key words】 Dwarfing and Early ripening Mutant RAPD Analysis Genetic difference;
【Key words】 Dwarfing and Early ripening Mutant RAPD Analysis Genetic difference;
【基金】 国家自然科学基金资助项目(A30170558)
- 【文献出处】 生物技术通报 ,Biotechnology Information , 编辑部邮箱 ,2005年02期
- 【分类号】S514
- 【被引频次】15
- 【下载频次】117