【摘要】 通过不同剂量的低能Ar+注入酿酒酵母Ke-y,筛选出了一种较好的菌体保护液,获得了一系列Ar+注入参数。在该研究体系内,Ar+最佳注入剂量分别为9 0×1015 ions/cm2 或13 5×1015 ions/cm2(适于IBB Device 1 型离子注入机)和1 0×1016ions/cm2 或1 4×1016 ions/cm2(适于LZD1000型离子注入机),为离子束介导外源基因组DNA在酵母菌Ke-y中的遗传转化奠定了基础。更多还原

【Abstract】 By the low energy Ar⁺ implantation of the different doses to Sacchromyces cerevisiae Ke-y,a kind of better organic solution as cell protector had chosen,and a series of implanting parameters had acquired.The best doese of Ar⁺ implantation to S.cerevisiae Ke-y were 9.0×10¹⁵ ions/cm² or 13.5×10¹⁵ ions/cm² suitable to IBB Device 1,and 1.0×10¹⁶ ions/cm² or 1.4×10¹⁶ ions/cm² suitabe to LZD 1000,which founded a base for ion beam mediated foreign genomic DNA genetic transformation in Sacchromyces cerevisiae Ke-y.更多还原