【摘要】 目的:通过检测重要的细胞凋亡调节因子Bax和Bcl-2的表达,探讨恒河猴胎盘细胞凋亡与药物流产的关系。方法:利用RT-PCR和原位杂交的方法检测恒河猴对照组和药物流产组(RU-486与AG诱导)胎盘中Bax和Bcl-2 mRNA的表达。结果:Bax mRNA在流产胎盘中的表达量明显高于对照组胎盘。在胎盘绒毛滋养层细胞可见明显表达,基底层蜕膜细胞也有表达。Bcl-2 mRNA在流产胎盘中的表达量明显低于对照组胎盘,表达部位与Bax类似。结论:RU-486和AG可能通过上调Bax mRNA和下调Bcl-2 mRNA的表达,导致胎盘组织凋亡细胞的数量明显增加,从而影响胎盘的正常结构和功能,增加了流产的危险性。更多还原

【Abstract】 Objective:To study the relationships between cellular apoptosis and abortion,the expression of Bax and Bcl-2 mRNA,the important apoptosis regulatory factors were detected.Methods:Bax and Bcl-2 mRNA were detected in placenta of control and treated groups(administrate RU-486 and AG),using RT-PCR and in situ hybridization.Results:The mRNA of Bax and Bcl-2 were identified obviously in the trophoblast cells of placenta and were visible in deciduas cells of basal line.The expression of Bax mRNA in the placenta of the control group was significantly lower than that of the treated group,whereas the expression of Bcl-2 mRNA in the placenta of control group was significantly higher than that of treated group.Conclusions:All the results suggested that the drugs might increase the quantity of apoptosis cells of placenta by upregulating the expression of Bax mRNA and downregulating the expression of Bcl-2 mRNA,which influence the natural structure and function of placenta,and the possibility of abortion increased.更多还原