【摘要】 目的 :通过普鲁兰 (pullulan)产生菌 -茁芽短梗霉 (AureobasidiumPullulans)原生质体的激光诱变 ,以得到普鲁兰高产菌株。方法 :利用正交实验研究了茁芽短梗霉原生质体的制备与再生并确定了其最佳条件为 :菌体以 1%的甘氨酸预处理 ;在 0 .1mol LpH 6 .0柠檬酸 -柠檬酸钠缓冲液 ,含 0 .7mol LNaCl的高渗稳定液中 ;经蜗牛酶 0 .2 %、纤维素酶 0 .1%、溶菌酶 0 .2 %的混合酶酶解 15min。采用He -Ne激光诱变茁芽短梗霉原生质体筛选得到普鲁兰高产菌株J2 0 8,其蔗糖转化率达到 5 3.3% ,是原始菌株的 10 .6倍。结论 :用激光诱变茁芽短梗霉原生质体是获得普鲁兰高产菌株的新途径更多还原

【Abstract】 Objectives:A high-yield pullulan strain was obtained by applying laser mutation technology on Aureobasidium Pullulans protoplast.Methods:Orthogonal experiment was used to confirm the optimum conditions of protoplast preparation and regeneration,which is the digestion of snailuse 0.2%,celluluse 0.1% and lysozyme 0.2% (30℃,15 min) after the strain was cultured for 40 min in Cit-NaCl high osmotic pressure liquid medium containing 1% Gly.He-Ne laser mutation method was used to treat protoplast of wild type strain and isolated a high-yeild mutant strain J208,whose transformation rate of sucrose is 53.3%,just 10.6 times as much as the original strain.Conclusion:Laser mutagenesis of Aureobasidium Pullulans protoplast is a new method to obtain high-yield pullulan strain.更多还原