【摘要】 采用甲酸脱氢酶(FateDH)、甲醛脱氢酶(FaldDH)和醇脱氢酶(ADH)3种脱氢酶为催化剂,以原型烟酰胺腺嘌呤二核甙酸(NADH)作为电子供体,通过3步连串反应将CO2转化为甲醇。采用正硅酸甲酯原位水解方法将二氧化硅掺杂于海藻酸(ALG)溶胶中,通过双交联制备出新型ALG-SiO2杂化凝胶。与ALG凝胶相比,ALG-SiO2结构更加紧密,酶的泄漏率大大降低,酶活性得以很好保持。将酶包埋于ALG-SiO2后,存放60 d以后或重复使用10次以上,酶活性都能保持80%以上,与之相比,包埋于ALG凝胶中的酶活性几乎完全丧失。更多还原

【Abstract】 Herein,CO₂ was converted into methanol through three step reduction reactions catalyzed by three different kinds of dehydrogenases,formate dehydrogenase(F_ateDH),formaldehyde dehydrogenase(F_aldDH) and alcohol dehydrogenase（ADH）.Reduced nicotinamide adenine dinucleotide （NADH） was employed as the electron donor.Alginate-silica(ALG-SiO₂) hybrid composite bead were prepared for the three dehydrogenases immobilization,through the hydrolysis and condensation of tetramethoxysilane（TMOS） in situ in an alginate solution,followed by Ca²⁺ crosslinking with blocks of guluronic acid residues.Compared to ALG gel,the ALG-SiO₂ composite was compact,and feasible for enzyme loading and enzymatic activity retention.After the ALG-SiO₂-enzyme biocomposite was stored for more than 60 days,or used for 10 cycles,more than 80% of the relative enzymatic activity could be retained.However,the relative enzymatic activity in the ALG-enzyme biocomposite was decreased almost to zero.更多还原