【摘要】 研究表明TGS往往与目的基因启动子的甲基化密切相关,RNA沉默则由目的基因的 mRNA特异性降解引起。植物体中诱导RNA沉默的外部因素有转基因和病毒。与传统的转基因 技术相比,病毒诱导的基因沉默(Virus-induced gene silencing VIGS)是一种瞬时表达体系,能在较 短的时间里取得良好的效果,目前被广泛地用来研究植物基因的功能。就VIGS的研究进展做一 个比较全面的综述。阐述了DNA和RNA病毒诱导植物基因沉默的机理,同时讨论了利用病毒诱 导的基因沉默(VIGS)鉴定植物基因功能的优缺点和将来的发展趋势。更多还原

【Abstract】 Plants recognize and degrade invading viral RNA homologous. Plant biologists adapted homology-based defense mechanisms triggered by incoming viruses to target individual genes for silencing in a process called virus-induced gene silencing (VIGS) .How DNA and RNA viruses have silence plant gene expression were examined and the advantages and disadvantages of VIGS in determining gene function and the future trend of virus induced gene silencing were discussed.更多还原