【摘要】 启动子是基因表达调控的重要顺式元件,也是基因工程表达载体的一个重要组成部分。启动子的克隆对于构建基因工程载体,表达目的蛋白有着重要的意义。启动子克隆的方法很多,从常用的利用启动子探针型载体筛选启动子到PCR方法的应用,此后相继问世的一些基于PCR法的克隆启动子技术,像I-PCR、P-PCR、SSP-PCR、YADE、TAIL-PCR等,为克隆启动子提供了更可靠,更合理的方法。对这几种方法进行了简要综述,比较了不同方法的优缺点,并展望了今后的研究前景。更多还原

【Abstract】 Promoter is an important cis-acting element, it is also an important element of gene engineering expression vectors. The way of promotor cloning is important for constructing gene engineering vectors and expressing the aim proteins. From the frequently used way that applying probe vectors for choosing promoter to the using of PCR, there were lots of ways for promotor cloning. Afterwards, a series of techniques based on PCR for promotor cloning such as I-PCR、P-PCR、SSP-PCR、YADE、 TAIL-PCR were developed in succession. They offered more reliable and reasonable ways of cloning the promoter. The ways of promoter cloning were briefly introduced. Meanwhile the limitation of these ways was introduced and their developing prospect was also discussed.更多还原