【摘要】 为了保留蜂毒肽抗类风湿关节炎活性,降低溶血性和致敏性,设计合成蜂毒肽的片段,观察它对兔免疫性关节炎的治疗作用。采用多肽合成法合成蜂毒肽结构中亲水性片段,经高效液相色谱(HPLC)及质谱(MS)分析。建立家兔卵蛋白性关节炎模型,进行溶血性试验、致敏性试验以及动物模型治疗实验。结果蜂毒肽片段溶血率<5%,致敏率<8%,动物模型治疗有效率可达到70%。蜂毒肽片段可有效地保留蜂毒肽抗类风湿关节炎活性,减低蜂毒肽的强溶血活性及致敏活性。更多还原

【Abstract】 To retain the anti-rheumatoid arthritis activity of melittin and to reduce the hemolysis and hypersusceptibility caused by melittin, a deletion peptide of melittin was synthesized.Its ant-inflammation effect was observed . A hydrophile peptide fragment of melittin was synthesized by standard solid-phase method.The product was analyzed by HPLC and MS. The relevant hemolysis and hypersusceptibility were tested .The rabbits’ model of immune arthritis were established and treated. The results showed that the hemolysis rate for peptide fragment was less than 5%, the hypersusceptibility rate was less than 8%. The hydrophile peptide fragment of melittin may retain anti-rheumatoid arthritis activity and reduce the melittin-induced hemolysis and hypersusceptibility.更多还原