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外消旋聚乳酸与软骨细胞体外培养的实验研究

Culture of Chondrocytes onto Poly-DL-Lactide Scaffoldings: Experimental Study

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【Author】 Peng Zhi 1# Song Yueming 1Δ Wang Dingguo2 Liu Limin1 Ding Yongli1 1(Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu 610041,China) 2(Sichuan Institute of Labour Hygiene and Occupational Disease, Chengdu 610041,China)

【机构】四川大学华西医院骨科；四川省劳动卫生职业病防治研究所；四川大学华西医院骨科成都610041；贵州省人民医院骨科； 550000；成都610041；成都610041；

【摘要】探讨国产外消旋聚乳酸(Poly-DL-Lactide,PDLLA)材料作为软骨组织工程技术中细胞培养支架的可行性及不同孔隙率的PDLLA支架对软骨细胞吸附增殖的影响。采用不同孔隙率的PDLLA支架与软骨细胞体外培养,观察其亲水性的改变,细胞吸附及对细胞增殖的影响。软骨细胞在不同规格的PDLLA支架上均能生长增殖分泌基质。孔隙率在90%~96%的PDLLA支架细胞吸附、增殖较佳。更多还原

【Abstract】 This experimental study was intended to find the possibility of using PDLLA as scaffolds in tissue engineering and to assess the effect of PDLLA scaffolds with different porosity on chondrocytes. Chondrocytes were cultured when they were seeded onto PDLLA scaffolds with different porosity. Under scanning electron microscope, the scaffolds in hydrophila and adsorptivity to chondrocytes and the function of the cells were observed. The chondrocytes were noted to proliferate on PDLLA scaffolds with different porosity, those with 90%-96% porosity being significantly better.So PDLLA can be used as a scaffold in tissue engineering, and the proper porosity of PDLLA scaffolds should be 90%~96%.更多还原

【关键词】支架；软骨；细胞培养；体外研究；
【Key words】 Scaffold Cell cultured Cartilage In vivo；

【基金】国家自然科学基金资助项目(39670224)

【文献出处】生物医学工程学杂志 ,Journal of Biomedical Engineering , 编辑部邮箱 ,2005年03期

【分类号】R329.2
【被引频次】1
【下载频次】101

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