【摘要】 通过观察频率、脉宽固定,电压峰值递增的脉冲电场对30只新西兰大白兔正常肝脏的杀伤效应,探讨在兔正常肝组织中建立脉冲电场分布模型的可行性,为临床电化学或电转基因治疗提供重要参考。采用重复实验及自身对照,脉冲处理3d后取材HE染色,光镜下见低脉冲剂量时两电极周围呈同心圆形坏死、界限清晰;剂量增大则同心圆形坏死区亦增大,且渐变成椭圆梭形,两电极中点处肝细胞出现核固缩、碎裂、溶解样改变,最后两椭圆梭形坏死相互重叠融合成不规则坏死,处理区尚见肝细胞的空化效应及肝组织缺血现象,实验所见与理论模拟的电场分布能较好吻合。因此,兔肝组织可作为脉冲电场生物组织杀伤效应研究中电场建模的有用载体,电脉冲在有效杀伤靶区组织的同时对周边正常组织是安全的。更多还原

【Abstract】 Pulsed electric fields(PEFs) with fixed frequency, width and gradually increased peak value of voltage was applied to 30 healthy rabbit liver tissues.The specific aims were to explore the feasibility of establishing a model of in vivo PEFs distribution in healthy rabbit liver tissues and to provide important references for clinical electrochemotherapy and for electrotransfer.Repeated experiment and self-comparison statistics design were implemented.The rabbit underwent the experiment under intravenous anesthesia and their liver tissues, after exposure to PEFs, were sent for HE staining.Necrotic borderline was visible 3 days after PEFs application,the necrotic shape of concentric circle was evident around the electrodes under optical microscope at lower voltage,as voltage increasing,two necroses in the shape of concentric circle gradually enlarged; nuclei with chromatin condensation,fragmentation and lysis alterations were seen in the middle region between the needles; concentric circles changed into ellipse fusiform and finally overlaped each other forming irregular necrosis contours.Cell cavitation and tissues ischemia were also observed within electric field. The shape of tissue necrosis from the experiment was noted to correlate with theoretic simulation of electric field distribution.Therefore, rabbit liver tissues can be a good carrier for in vivo modeling of electric field distribution when the lethal effects of PEFs in tissues are investigated. PEFs also show safety for the surrounding normal tissue while causing damage or injury to the target area therapeutically.更多还原