【摘要】 制备聚丙交酯/乙交酯/天冬氨酸-聚乙二醇(PLGA-[ASP-PEG])多元共聚物并检测其细胞相容性,为骨组织工程生物材料的研制和改进提供实验依据。通过本体开环共聚方法合成PLGA-[ASP-PEG]多元共聚物,进行红外光谱(IR)和核磁共振氢谱(1HNMR)分析;以PLGA作对比,分别复合骨髓基质细胞(Bonemarrowstromalcells,BMSCs)进行体外培养,进行相差显微镜和扫描电镜观察。IR和1HNMR证明PLGA-[ASP-PEG]多元共聚物形成;BMSCs在PLGA-[ASP-PEG]表面的黏附、扩展和增殖情况均优于PLGA。PLGA-[ASP-PEG]具有良好的细胞相容性,有望成为骨髓基质细胞的载体应用于骨组织工程。更多还原

【Abstract】 To prepare poly(lactic acid/glycolic acid/ asparagic acid-co- polyethylene glycol)(PLGA-[ASP-PEG]) and examine the cellular biocompatibility. PLGA-[ASP-PEG] was obtained by bulk ring-opening copolymerization method, examined by infrared spectrometry(IR) and 1H nuclear magnetic resonance spectroscopy(1H NMR). Bone marrow stromal cells(BMSCs) were cultured with PLGA-[ASP-PEG](experiment gruop) and PLGA(control group) in vitro respectively, and were observed by phase-contrast microscopy and scanning electron microscopy. The resuls showed that PLGA-[ASP-PEG] was obtained and proved by IR and 1H NMR. The BMSCs of the experiment group could well attach to and extend on the surface of the PLGA-[ASP-PEG], and could proliferate and secrete better extracellular matrix, compared with control. The PLGA-[ASP-PEG] has good cellular a biocompatibility. It can be used as a biomaterial for bone tissue engineering.更多还原