【摘要】 探讨成骨细胞和脱蛋白骨-钛网(DPB- TM)支架复合物修复骨缺损的能力。用新西兰兔30只,随机分成3组,各10只。实验组第三代胎兔成骨细胞以5×10 6 m l- 1浓度接种于DBP- TM支架中,复合物体外培养1周后修复颅骨1.5 cm×1.5 cm缺损。对照组 、,同样的缺损分别用DBP- TM支架修复和注射成骨细胞悬液。术后12周,三维CT检查后,对取下的标本进行大体、扫描电镜、组织学观察及生物力学测试。结果显示,实验组植入的复合物与正常骨组织之间分界线模糊,有骨小梁通过,界面紧密融合;支架上有大量新骨形成,且支架部分被吸收;植入12周后的复合体抗压强度(18.93±1.12 MPa)大于正常颅骨(16 .96±1.6 0 MPa) (P<0 .0 5 )。对照组均无新生骨,仅见缺损区有纤维样组织覆盖。因此,体外培养的成骨细胞经增殖后接种于DPB- TM支架中可以用来修复骨缺损。更多还原

【Abstract】 To investigate the ability of composite graft of osteobals ts and deport einized bone-titanium mest(DPB-TM) scaffold to repair cranial bone defect. 30 rabbits were randomly divided into 3 groups. The passage 3 fetal rab bit osteoblasts were seeded into porous DPB-TM scaffolds at the density of 5×1 0 6ml -1 as the experimental group. The same defects were respectively rec onstructe d by DPB-TM or osteoblasts as the control groups. After 12 weeks, the result wa s evaluated by three-dimensional computed tomographic scanning,gross inspection, scanning electron microscopy, histological examination and mechanics test, resp e ctively. In the experimental group, bone trabecula was observed to pass the defe ct and interface was mixed. No demarcation between the region of the bone defect and the normal bone was observed. There was plenty of new bone on the scaffold. Part of the scaffold was absorbed. In view of mechanics, the intensity of artificial bone (1 8.93±1.12 MPa) was higher than that of normal bone (16.96±1.60 MPa) (P<0. 05) . In the control groups, only fibrous tissue was observed in the defect region, there was no new bone formation. The tissue engineering bone constructed by osteoblasts and DPB-TM scaffold can be applied to the repair of bone defect.更多还原