【摘要】 海南五指山猪 (Wuzhishanpig)是我国珍稀的小型猪品种。它的解剖学、生理学、疾病发生机理等都与人类极其相似 ,具有重要的应用价值 ,可用于心血管病、皮肤烧伤和新药评价等许多方面的研究。作者利用多重PCR和基因扫描技术对五指山猪 32个微卫星基因座进行了遗传检测。统计了全群、核心群和近交群的等位基因组成 ,并计算了它们的平均纯合率、多态信息含量 (PIC)和平均杂合度。结果显示 ,全群、核心群和近交群 32个基因座的平均等位基因数分别为 14 .5 90个、9.4 10个、13.6 6 0个 ,平均基因纯合率分别为 4 4 %、4 4 .3%、4 4 % ,平均PIC分别为0 .74 2、0 .70 8、0 .731,平均杂合度分别为 0 .5 6 0、0 .5 5 8、0 .5 5 9。这表明海南五指山猪虽然遗传基础比较稳定 ,但仍具有较大的遗传变异。上述结果对于海南五指山猪的保种、定向选育、开发利用都具有重要的指导意义更多还原

【Abstract】 Wuzhishan pig, which originated from Hainan Province, China, is one of China′s rare miniature pig breeds. It is a potential genetic resource for sustainable pig production. In addition, it can be utilized as an experimental animal for medical and veterinary research due to its small size with the mature body weight of only about 25 kg. We detected its genetic polymorphisms using 32 microsatellites distributed on 14 chromosomes. A total of 200 individuals were sampled from the nuclear herd and from an inbred herd at the Wuzhishan Pig Breeding Farm in Hainan Province and analysed by multiplex PCR and gene scanning techniques. The average numbers of alleles at the 32 marker loci were 9.410, 13.660, and 14.590 in the nuclear group, the inbred group, and the merged group, respectively. The average homozygosity was almost the same in the three groups, about 44% in each case. The polymorphism information content in the three groups was 0.708, 0.731, and 0.742, respectively. The results indicate a rich genetic polymorphism in Wuzhishan pig.更多还原