【摘要】 通过在培养基中加入一定浓度的Cu(CuSO4·5H2 O ,Cu 2 0 0mg·L-1) ,从 4 2份土壤样品中筛选得到 2株具有很强Cu抗性的细菌B和G ,经初步鉴定它们分别属于无色细菌属和芽胞杆菌属。将菌株B和G接种到添加Cu(15 0mg·L-1)的培养液中进行沉淀态铜的活化试验 ,结果表明 ,与不接菌对照相比 ,B和G菌株分别使培养液中有效铜含量增加 4 75 5 %和 2 4 9 5 % ,培养液pH由 6 5分别降低到 4 2和 5 0 ,菌株B和G的活化效能与其代谢产酸有关。B菌株和G菌株最适生长温度为 2 8℃ ,最适 pH分别为 6～ 7和 8,G菌株在 5 %NaCl下生长良好。除抗Cu外 ,两株菌对重金属Pb(40 0mg·L-1)、Cd(10 0mg·L-1)也具抗性。更多还原

【Abstract】 Copper-resistance (Cu 200 mg·L -1 ) strains B and G were screened out from 42 soil samples,which were primarily identified as Achromobacter and Bacillus.Strain B and G were inoculated to the solution containing Cu (150 mg·L -1 ) for activation tests.The results showed that in the liquid medium inoculated strain B and G,the contents of Cu 2+ increased by 475.5% and 249.5% respectively compared with the control,and pH decreased from 6.5 to 4.2 and 5.0,respectively.The activation efficiency of the bacteria correlated to their ability to produce acid.The optimum growth conditions for them were: temperature 28 ℃ for both strain B and G,pH 6～7 for strain B and 8 for strain G.Strain G grew well in the medium containing 5% NaCl.Moreover,strain B and G can also be resistant to Pb 2+ (400 mg·L -1 ) and Cd 2+ (100 mg·L -1 ).更多还原