【摘要】 斑鳠是珠江四大名鱼之一,目前资源量急剧下降,种质资源的保护迫在眉睫。测定了斑鳠线粒体细胞色素b 基因的全序列,该序列全长1 137 bp,其中T、C、A、G四种碱基含量分别为31.6%、25.7%、29.6%、13.1%,A+T 的含量(61.2%)显著高于G+C的含量(38.8%)。将测定序列(序列自编号:MGGD)与GenBank中的2个斑鳠序列(序列自编号:MGZJ、MGFJ)进行了比较,3个序列之间的序列差异为0.005-0.017,转换后的氨基酸序列间差异位点都仅为2 个,反应出细胞色素b基因作为功能蛋白质编码基因序列相对比较保守。鳠属鱼类线粒体Cytb基因的转换大于颠换。研究为斑鳠渔业种质资源保护和品种选育提供了遗传方面的资料,斑鳠的群体结构有待进一步深入研究。更多还原

【Abstract】 Mystus guttatus is one of four famous fishes m the Pearl River, but the population depression is repaidly rapidly in recent years, and fishing resource needs protection. 1137 nucleotides of mitochondrial Cytochrome b gene sequence(number: MGGD) have been obtained from Chinese bagrid fish Mystus guttatus, the contents of T、C、A、G of the gene are 31.6%、25.7%、29.6 %、13.1%respectively. Respectively compared to other two sequences from GenBank(number: MGZJ,MGFJ), the gene sequence divergence of M guttatus among the three sequences is 0.005 to 0.017. The difference of amino acid sequences converted by gene sequences are all only 2 sites, so it shows that Cytb gene which acts as a functional gene is conservative. And value of transition is larger than value of transversions in Mystus fishes. The research is aimed to offer some genetic data of M guttatus for fishing resource protection and genetic selection, and the structure of Mystus guttatus population need be further researched.更多还原