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非晶颗粒态玉米淀粉不同条件下酶降解活性研究
Study on α-amylase degraded activity of corn starch with non-crystallized granule state under different conditions
【摘要】 采用光学显微镜分析测试方法,对非晶颗粒态玉米淀粉不同条件下酶降解活性进行了观察和研究,结果发现,α-中温淀粉酶生物活性对非晶颗粒态淀粉作用能力高,使用较少量的酶作用于非晶颗粒淀粉,在颗粒表面上可以观察到明显变化.非晶颗粒态淀粉颗粒由于存在爆裂口,所以酶对非晶淀粉作用均从爆裂口开始,逐渐由爆裂口开始均匀扩张,颗粒模糊,最后颗粒消失,不同时间、酶量、温度对酶降解作用均产生一定影响.其中温度可明显加快酶降解速度,酶量的增加同样对酶降解产生显著影响.时间的延长也可以使非晶颗粒态淀粉逐渐降解,但变化较慢.
【Abstract】 Using polarized light microscope analytical method,a-amylase degraded activity of corn starch with non-crystallized granule state under different conditions was investigated.It was found that a-amylase(medium temperature) has strong degraded ability to non-crystallized starch,a little enzymes were used to non-crystallized starch,visible changes were observed on the surface of granules.From the beginning of blowing out poles of starch granules,starch granules were degraded deeply by α-amylase along blowing out poles to completely disappear.Different time,amylase amount and temperature had some affects on a-amylase degraded activity.Degraded activity can be speeded by higher temperature.And increasing amount of a-amylase was the same way as high temperature.Prolonging react time can made non-crystallized corn starch degrade,but the speed of change was slow.
【Key words】 corn starch; non-crystallized granule state; α-amylase degrade activity;
- 【文献出处】 商丘师范学院学报 ,Journal of Shangqiu Teachers College , 编辑部邮箱 ,2005年05期
- 【分类号】TS231;
- 【被引频次】17
- 【下载频次】171