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非晶颗粒态玉米淀粉不同条件下酶降解活性研究

Study on α-amylase degraded activity of corn starch with non-crystallized granule state under different conditions

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【作者】 梁勇张本山杨连生高大维

【Author】 LIANG Yong~1,ZHANG Ben-shan~2,YANG Lian-sheng~2,GAO Da-wei~2(1.Dept.of Chemistry,South China Normal University,Guangzhou 510641,China;2.Light and Chemical Industry Institute,South China University of Technology,Guangzhou 510641,China)

【机构】 华南师范大学化学系华南理工大学轻化所华南理工大学轻化所 广东广州510641广东广州510641广东广州510641

【摘要】 采用光学显微镜分析测试方法,对非晶颗粒态玉米淀粉不同条件下酶降解活性进行了观察和研究,结果发现,α-中温淀粉酶生物活性对非晶颗粒态淀粉作用能力高,使用较少量的酶作用于非晶颗粒淀粉,在颗粒表面上可以观察到明显变化.非晶颗粒态淀粉颗粒由于存在爆裂口,所以酶对非晶淀粉作用均从爆裂口开始,逐渐由爆裂口开始均匀扩张,颗粒模糊,最后颗粒消失,不同时间、酶量、温度对酶降解作用均产生一定影响.其中温度可明显加快酶降解速度,酶量的增加同样对酶降解产生显著影响.时间的延长也可以使非晶颗粒态淀粉逐渐降解,但变化较慢.

【Abstract】 Using polarized light microscope analytical method,a-amylase degraded activity of corn starch with non-crystallized granule state under different conditions was investigated.It was found that a-amylase(medium temperature) has strong degraded ability to non-crystallized starch,a little enzymes were used to non-crystallized starch,visible changes were observed on the surface of granules.From the beginning of blowing out poles of starch granules,starch granules were degraded deeply by α-amylase along blowing out poles to completely disappear.Different time,amylase amount and temperature had some affects on a-amylase degraded activity.Degraded activity can be speeded by higher temperature.And increasing amount of a-amylase was the same way as high temperature.Prolonging react time can made non-crystallized corn starch degrade,but the speed of change was slow.

【基金】 国家自然科学基金资助项目(29976016)
  • 【文献出处】 商丘师范学院学报 ,Journal of Shangqiu Teachers College , 编辑部邮箱 ,2005年05期
  • 【分类号】TS231;
  • 【被引频次】17
  • 【下载频次】171
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