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黑木耳多糖酶法提取条件的研究

STUDIES ON THE EXTRACTIVE CONDITIONS OF AURICULARIA AURICULAR POLYSACCHARIDE IN ENZYMATIC METHODS

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【作者】 张立娟于国萍周国华

【Author】 ZHANG Lijuan YU Guoping ZHOU Guohua,Food Science College,Northeast Agricultural University,Harbin,150030

【机构】 东北农业大学食品学院东北农业大学食品学院 哈尔滨150030哈尔滨150030哈尔滨150030

【摘要】 对不同条件下黑木耳多糖酶法提取的影响因素进行了研究,结果表明,利用酶法结合热水浸提法提取黑木耳多糖,提取率达17.2%。酶解最适作用条件为:复合酶反应的最适pH值为5,添加量为1.5%,反应100min;蛋白酶反应的最适pH值为6,添加量为2%,反应60min,80℃浸提90min。

【Abstract】 We study the influence factors of extraction on Auricularia auricular polysaccharides in enzymatic methods under different conditions. The result is that the extractive rate is 17.2% when extracting auricularia auricular polysaccharides in enzymatic and hot water soaking method. The best conditions of enzymatic action is that the best pH of complex enzyme is 5 and the amount is 1.5% and reacting time is 100min, and the best pH of protease is 6.5 and the amount is 2% and reacting time is 60min,soaking 90min in hot water at 80℃.

【关键词】 黑木耳多糖蛋白酶纤维素酶果胶酶提取
【Key words】 Auricularia auricularpolysaccharidesproteasecellulasepectaseextraction
【基金】 黑龙江省教育厅重大研究项目,项目号:10511Z006
  • 【分类号】TS219
  • 【被引频次】58
  • 【下载频次】789
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