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低聚甘露糖的酶法制备、分离与结晶

Preparation of Mannooligosaccharides by the Enzymatic Method and Its Separation as Well as Crystallization

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【作者】 李道义江正强韦赟李里特日下部功

【Author】 LI Dao-yi,JIANG Zheng-qiang,WEI Yun,LI Li-te,KUSAKABE Isao(College of Food Science and Nutriltional Engineering, China Agricultural University, Beijing 100083, China)

【机构】 中国农业大学食品科学与营养工程学院中国农业大学食品科学与营养工程学院 北京100083北京100083

【摘要】 利用枯草芽孢杆菌WY-45所产甘露聚糖酶水解椰子壳甘露聚糖,产物以低聚甘露糖为主,得率为44%。所得糖液采用活性炭柱层析,分离纯化结晶,得到了S1-S4四种糖晶体,经鉴定为甘露二糖、甘露三糖、甘露四糖和半乳甘露四糖。

【Abstract】 Copra mannan was hydrolyzed by the crude β-mannanase from Bacillus subtilis WY-45 to yield mannooligosaccharidesas the main products, and the yield was up to 44%. The hydrolyzate was separated by active carbon column chromatography,and four kinds of mannooligosaccharides were obtained as crystalline state. They were identified as manobiose, mannotriose,mannotetrose andg alactosylmannotetrose, respectively.

  • 【分类号】TQ28
  • 【被引频次】11
  • 【下载频次】731
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