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低聚甘露糖的酶法制备、分离与结晶
Preparation of Mannooligosaccharides by the Enzymatic Method and Its Separation as Well as Crystallization
【摘要】 利用枯草芽孢杆菌WY-45所产甘露聚糖酶水解椰子壳甘露聚糖,产物以低聚甘露糖为主,得率为44%。所得糖液采用活性炭柱层析,分离纯化结晶,得到了S1-S4四种糖晶体,经鉴定为甘露二糖、甘露三糖、甘露四糖和半乳甘露四糖。
【Abstract】 Copra mannan was hydrolyzed by the crude β-mannanase from Bacillus subtilis WY-45 to yield mannooligosaccharidesas the main products, and the yield was up to 44%. The hydrolyzate was separated by active carbon column chromatography,and four kinds of mannooligosaccharides were obtained as crystalline state. They were identified as manobiose, mannotriose,mannotetrose andg alactosylmannotetrose, respectively.
【关键词】 低聚甘露糖;
β-甘露聚糖酶;
枯草芽孢杆菌;
制备;
结晶;
【Key words】 mannooligosaccharides; β-mannanase; Bacillus subtilis; preparation; crystallization;
【Key words】 mannooligosaccharides; β-mannanase; Bacillus subtilis; preparation; crystallization;
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2005年S1期
- 【分类号】TQ28
- 【被引频次】11
- 【下载频次】731