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菠菜叶蛋白的提取研究

Study on the Extraction of Spinach Leaf Protein

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【作者】 舒友琴梁丽琴扶庆权王伟邱伙琴李国媛袁道强

【Author】 SHU You-qin1,LIANG Li-qin2,FU Qing-quan2,WANG Wei2,QIU Huo-qin2, LI Guo-yuan2,YUAN Dao-qiang2 (1.Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450002, China;2.School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China)

【机构】 郑州牧业工程高等专科学校郑州轻工业学院食品与生物工程学院郑州轻工业学院食品与生物工程学院 河南郑州450002河南郑州450002河南郑州450002

【摘要】 测定不同生长时期内的菠菜叶蛋白含量,以高蛋白含量生长期内的干菠菜叶作为实验材料,研究不同pH值、提取温度、提取时间及料液比对菠菜叶蛋白溶出率的影响。结果表明,pH值9.5、提取温度55℃、提取时间60min、料液比1:35为菠菜叶蛋白的最佳提取条件;pH4.0为菠菜叶蛋白的最佳沉淀pH值。

【Abstract】 The protein contents in different growth periods of the spinach leaves were assayed, and the dry spinach leaves of high protein contents during growth period were used as the raw material of this experiment.The effects of pH value,extracting temperature,extracting time and the ratio of water in extracting medium were studied. The results showed that pH9.5, extractingtemperature 45℃, extracting time 60min and the ratio of extracting medium to water 1:35 were the optimum extracting conditions of spinach leaf protein; while pH4.0 was the optimum precipitation pH value.

【关键词】 菠菜叶蛋白提取率
【Key words】 spinach leaf proteinextraction
【基金】 河南省科技攻关项目(0424290041);郑州市重大科技攻关项目(2002BA02)
  • 【分类号】TQ936.1;
  • 【被引频次】33
  • 【下载频次】539
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