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反相液相色谱法测定芦荟制品中芦荟苷的含量

Determination of Aloin in Aloe Products by RP-HPLC

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【作者】 龙洲雄万春花胡海山王凯张云伟

【Author】 LONG Zhou-xiong,WAN Chun-hua,HU Hai-shan,WANG Kai,ZHANG Yun-wei(Jiangxi Institute of Analysing and Testing, Nanchang 330029, China )

【机构】 江西省分析测试研究所江西省分析测试研究所 江西南昌330029江西南昌330029江西南昌330029

【摘要】 采用甲醇直接超声提取、反相液相色谱法测定芦荟制品中芦荟苷的含量。使用KromasilC18柱,乙腈:水(体积比25:75)为流动相,流速1.0ml/min,波长359nm。方法平均回收率97.8%,RSD在0.75~3.8%(n=5)之间。本法简便、快速、准确可靠。

【Abstract】 This paper reported a method for determining aloin in aloe products by RP-HPLC. After being extracted with methanolby ultrasonic, aloin was separated on a Kromasil C18 column with acetonitrile-water(25:75 by volume) as a mobile phase at a flowrate of 1.0ml/min and detected at 359nm. The average recovery was 97.8% and the relative standard deviations(n=5) was 0.75~3.8%. This method was proved to be simple,rapid,accurate and reliable.

【关键词】 反相液相色谱法芦荟制品芦荟苷
【Key words】 RP-HPLCaloe productAloin
  • 【分类号】TS207.3
  • 【被引频次】5
  • 【下载频次】168
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