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免疫初乳中IgG的分离与纯化

Isolation and Purification of IgG from Immune Colostrums

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【作者】 范丽娟潘道东

【Author】 FAN Li-juan1,PAN Dao-dong2,* (1.Life Science College, Nanjing Normal University, Nanjing 210097, China;2.Food Science and Nutrition Department, Ginling Women’s College, Nanjing Normal University, Nanjing 210097, China)

【机构】 南京师范大学生命科学学院南京师范大学金陵女子学院食品科学与营养系 江苏南京210097江苏南京210097

【摘要】 本实验采用硫酸铵一次性盐析、DEAE-SepharoseFF离子交换层析和SephacrylS-200凝胶过滤层析法,分离纯化免疫初乳中的IgG,并采用SDS-PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测,结果表明所得IgG纯度高,活性大。

【Abstract】 IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased.

【关键词】 免疫初乳IgG分离纯化
【Key words】 immune colostrumsIgGisolation and purification
【基金】 国家十五奶业重大科技专项(2002BA518A12);江苏省十五攻关项目(BE2002302);江苏省自然科学基金(BK2005137)
  • 【分类号】TS252.59
  • 【被引频次】11
  • 【下载频次】345
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