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免疫初乳中IgG的分离与纯化
Isolation and Purification of IgG from Immune Colostrums
【摘要】 本实验采用硫酸铵一次性盐析、DEAE-SepharoseFF离子交换层析和SephacrylS-200凝胶过滤层析法,分离纯化免疫初乳中的IgG,并采用SDS-PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测,结果表明所得IgG纯度高,活性大。
【Abstract】 IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased.
【基金】 国家十五奶业重大科技专项(2002BA518A12);江苏省十五攻关项目(BE2002302);江苏省自然科学基金(BK2005137)
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2005年08期
- 【分类号】TS252.59
- 【被引频次】11
- 【下载频次】345