【摘要】 采用大孔树脂柱层析分离得到大豆皂甙SS-Ⅰ、SS-Ⅱ、SS-Ⅲ三个级分,利用高效液相色谱-电喷雾负离子串联质谱对SS-Ⅲ进行了详细研究。结果表明:在线的高效液相色谱-电喷雾负离子一级质谱提供了准分子离子峰,二级质谱出现大量的糖链碎片信息,通过分析确定SS-Ⅲ中含有四种B组大豆皂甙,一种E组大豆皂甙,并发现一种未见报道的E组皂甙。更多还原

【Abstract】 Soyasaponins of three fractions(SS-Ⅰ、SS-Ⅱ、SS-Ⅲ) were obtained by D101A macroporous resin column chromatographic method. SS-Ⅲ were determined by high performance liquid chromatography-negative electrospray ionization tandem mass spectrometry(HPLC-ESI-/MS2).In the method, on line HPLC-ESI-/MS allowed to obtain rapidly useful information about the molecular weight while HPLC-ESI-/MS2 offered the abundant fragment ion peak. So four known group B soyasaponins and one group E soyasaponin were determined respectively. One new composition that had not been reported in soyasaponins before was identified.更多还原