【摘要】 采用两倍稀释法在脱脂乳培养基和乳清培养基中测定了nisin对嗜热链球菌9的最小抑菌浓度(MIC)。在脱脂乳培养基中逐渐增加nisin的浓度,驯化嗜热链球菌9,直到其抗性达到300IU/ml。经生产性能鉴定和遗传性状实验,获得两株适宜做酸奶发酵剂的nisin抗性嗜热链球菌9.31和9.4。更多还原

【Abstract】 The MIC of nisin to Streptococcus thermophilus was determined by serial twofold dilutions in skim milk medium and whey medium. In skim milk medium, Streptococcus thermophilus 9 was tamed by gradually increasing the nisin concentration until its resistance reaching 300 IU of nisin per ml. The two nisin-resistant Streptococcus thermophilus strains 9.31 and 9.4, fit for two yogurt culture were gained and assayed by production performance identification and genetic stability test.更多还原