【摘要】 以米根霉AS3.819的诱变株为菌种,甘薯淀粉为碳源,在摇瓶发酵工艺的基础上,利用10L发酵罐对该菌株产L-乳酸进行研究,探索出10L罐最适发酵条件为:装液量40%,搅拌转速400～650r/min,通气流量0.5～0.6L/(L·min),溶氧≥88%,温度32℃,pH5.0～5.5,消泡剂添加量0.1%左右。罐发酵结果得到L-乳酸累积浓度为99.54g/L,对原淀粉的转化率达77%,最高生物量7.2g/L,发酵周期70h。更多还原

【Abstract】 A 10L jar fermentation technology for production of L-lactic acid from sweet potato starch incubated with a mutant of Rhizopus oryzae AS 3.819 was developed. The optimal culture conditions were as follows: 10L jar contained 4L liquid culture medium with rotational speed from 400 to 650r/min, aeration rate from 0.5 to 0.6L/(L·min), dissolved oxygen (DO) above 88%, fermentation temperature 32℃, pH from 5.0 to 5.5 and the dose of defoamer 0.1%. Results indicated that the average yield of L-lactic acid, the conversion rate of crude starch to L-lactic acid and top biomass fermentation time were 99.54g/L, 77%, 7.2g/L and 70h, respectively.更多还原