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产β-半乳糖苷酶酵母菌株15D产酶条件研究
Study on the conditions for the production of β-galactosidase by yeast 15D
【摘要】 从马克斯克鲁维酵母的突变菌株中,获得了一株高产β-半乳糖苷酶的酵母菌株15D。本文对菌株15D的产酶条件及小型发酵培养实验进行研究,表明在适宜条件下菌株15D产酶水平可达287.8U/mL,比出发菌产酶水平提高了5倍。
【Abstract】 The yeast 15D strain with high yield of β-glycosidase was obtained from Kluyveromyces marxianus Var.marxianus by mutation with common screening tests. This paper studies the conditions for the production of β-galactosidase by the yeast 15D and it’s minitype fermentation experiment. Under optimum conditions enzyme production could reach 287.8U/mL, which is 5 times higher that of original strain.
【关键词】 β-半乳糖苷酶;
酵母;
液体发酵;
产酶条件;
【Key words】 β-glycosidase; yeast; liquid state fermentation; conditions of producing enzyme;
【Key words】 β-glycosidase; yeast; liquid state fermentation; conditions of producing enzyme;
【基金】 甘肃省科技厅资助项目(GKS002-B01-01)。
- 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2005年12期
- 【分类号】TS201.25
- 【被引频次】3
- 【下载频次】140