固定化乳酸乳球菌DL-203发酵生产Nisin的研究

【摘要】 对用固定化乳酸乳球菌DL-203发酵生产Nisin进行了研究。确定采用k-卡拉胶作为固定化载体,并添加磷酸三钙,包埋固定化乳酸乳球菌DL-203发酵生产Nisin。固定化最适条件为3.5%(W/V)k-卡拉胶+0.1%(W/V)磷酸三钙,细胞浓度5%～10%(V/V)。发酵培养基添加0.2%(V/V)吐温-20,30℃摇床培养进行分批重复发酵,可连续使用五批以上,而发酵液Nisin效价未有下降,达游离细胞发酵液的50%～60%。采用柱式填充床反应器进行固定化乳酸乳球菌DL-203连续发酵生产Nisin,停留时间以4～6h比较适宜,可连续稳定生产150h以上,而且固定化小球未有明显的细胞外漏,发酵液Nisin效价可达游离细胞发酵液的30%～40%。更多还原

【Abstract】 In this paper, the production of Nisin with immobilized Lactococcus lactis subsp. lactis DL-203 has been studied and the optimal conditions for immobilization has been designed: 3.5%(W/V) k-carrageenan including 0.1%(W/V) tricalcium phosphate with DL-203 cultures (55%～10%,V/V) to prepare immobilized cells. When the fermentation medium was added to 0.2%(V/V) Tween-20, the production of Nisin with immobilized cells was 50%～60% of that of the free cell fermentation during batch fermentation. Continuous production of Nisin with immobilized cells was carried on in colum packed bed reactor. It was found that the optimum retention time was 4～6 hours and the production of Nisin which was about 30%～40% of that of the free cell fermentation remained stable for more than 150 hours.更多还原