菜籽肽清除自由基作用的研究

【摘要】 双酶分步水解的菜籽清蛋白粗品肽(RSP-R)经SephadexG-25凝胶层析柱分离纯化,得到分子量依次降低的三个级分(RSP-1,RSP-2和RSP-3)。离体实验结果表明,菜籽肽具有较强的还原能力及抑制·OH的作用;RSP-R和RSP-1对活性氧的产生有抑制作用,抑制效果的最佳浓度为0.5mg/mL。更多还原

【Abstract】 The rapeseed peptides (RSP-R) ,produced with Alcalase and Flavourzyme, was separated by Sephadex G-25 gel filtration chromatography, and three parts of peptides (RSP-1RSP-2 and RSP-3) were obtained. The results in vitro showed that RSP had higher reductive activity and can scavenge hydroxyl radicals(·OH). As for the inhibiting effect on the generation of reactive oxygen species (ROS), RSP-R and RSP-1 was better than others, the optimum concentration was 0.5mg/mL.更多还原