【摘要】 探讨了经80℃热水提取、Sevag法除蛋白、乙醇分级沉淀等步骤提取刺五加多糖,并应用苯酚-硫酸法测定其含量。本法选取490nm为反应产物的测定波长,所测葡萄糖标准曲线相关系数r=0.99956,线性范围为5-22.5μg/mL,测得多糖的总收率为刺五加干重的0.54%。本法反应产物在160min内吸光度值稳定,RSD为1.22%,精密度良好,回收率为97.6%-102.8%。更多还原

【Abstract】 This paper studies the extraction and analysis of polysaccharides from Acanthopanax senticosus, the processing steps are as follows: extract the polysaccharides from Acanthopanax senticosus by 80℃ water at first, then deproteinize according to the method of Sevag, and then precipitate the polysaccharide by ethanol. Phenol-sulfuric acid assay is applied to determinate the polysaccharides content quantitatively. In this method, we adopt 490nm as the optimum wavelength for determination, the relative coefficient of the standard curve of glucose is 0.99956, the linear range is 5-22.5 μg/mL The total yield is 0.54% of the desiccated Acanthopanax senticosus. The absorbance of the product doesn’t change in 160 minutes, i.e. product is quit stable. The precision is good, the RSD is not more than 1.22%, and the range of recovery is 97.6% -102.8%.更多还原