【摘要】 明确了假蜜环菌试验菌株摇瓶培养条件为:碳源浓度25g/L,氮源浓度0.336g/L,初始pH4.0,种龄为7d的液体种子接种量为10%(V/V),在27℃,摇床转速140r/min。对照此培养条件,确定假蜜环菌深层发酵培养基的组成为:经过破壁处理啤酒废酵母粉4.8g/L,葡萄糖24g/L。相同培养条件下,菌丝体收获量为干重13.5g/L,生物量高于利用蛋白胨培养基扩大培养的产量:13.0g/L。更多还原

【Abstract】 Cultivation of Armillariella mellea with used brewer’s beer yeast was studied. The result shows the optimum cultivation conditions for Armillariella mellea in shaking flask. The optimum nitrogen concentration was 25g/L, the optimum carbon concentration was 25g/L, the initial pH was 4.0, the optimum temperature was 27℃, optimum seed age was 7 days, inoculum concentration was 10%, and the rotary speed was 140r/min. Based this condition, the composition of the culture medium was as follows: dried brewer’s yeast 4.8g/L, glucose 24g/L. 13.5g biomass was obtained with the medium, while 13.0g/L biomass was obtained with peptone medium.更多还原