【摘要】 用不同参数的变幅杆浸入式超声波作用酵母细胞,测定细胞内核酸、蛋白质及FDP(1,6-二磷酸果糖)3种物质渗出率,再用透射电镜观察细胞形态。探讨超声场对酵母细胞膜通透性影响的最佳参数及机理。结果表示,超声场的最佳参数是:电功率500W、脉冲时间3s、间隙时间4s、脉冲总时间共225s,影响酵母细胞膜通透性变化的主要机制是空化作用使细胞膜在一定程度上损伤产生穿孔效应,细胞膜的通透性提高。更多还原

【Abstract】 The release of nucleic acid, protein and FDP were determined on Saccharomyces cerevisiae cell by different parameters of ultrasound. The cell configuration was assayed by transmission electron microscopy (TEM). The aim of this paper was to study the optimal parameters and mechanism of Saccharomyces cerevisiae cell membrane permeability induced by ultrasound. The optimal parameters were power 500 W, treating time 225s pulse 3s and interval time 4s, respectively. The mechanism of ultrasound induced cell membrane permeability was that the cavitation effect of ultrasoud led to the perforated effect resulted from cell membrane damage. Ultrasoud could increase the permeability of Saccharomyces cerevisiae membrane.更多还原