【摘要】 在螺旋藻生长的不同阶段分次添加无机硒(Na2SeO3)进行富硒处理。设置11个实验组,分别从第1天至第10天分次加硒,观察不同硒处理组中螺旋藻对无机硒的富集转化及其光合色素、蛋白质的变化情况。结果表明,在螺旋藻生长的第7、8、9三天分次加硒(实验组7),可获得硒产率1743.06μg/L、有机硒比率为86.85%的高富硒量螺旋藻,与对照相比,其硒含量增加150倍,生物量也明显提高(1.34g/L,对照组为1.18g/L);该实验组藻体中的硒主要分布在水溶性蛋白质中,占总硒含量的60.51%,占有机硒含量的69.67%;螺旋藻主要营养物质藻蓝蛋白(PC)、别藻蓝蛋白(APC)、类叶黄素、β-胡萝卜素含量也有不同程度的增加,表明此富硒方法是螺旋藻高值化处理的有效途径。更多还原

【Abstract】 Effects of different selenium (Se) adding methods on the growth, Se accumulation and transformation of Spirulina platensis were investigated. The results indicated that the high Se-enriched S. platensis with a total Se content of 1301.17 μg/g and an organic ratio of 86.85 % could be obtained while the Se was added in the 7 th , 8 th , 9 th day with an accumulative concentration of 1 000 mg/L. A stimulative effect in biomass of Se-enriched cultures was observed in the 7th experimental set, comparing with the control cultures. Furthermore, the contents of phycobiliproteins and photosynthetic pigments (Lutein and β-carotene) increased to some extend. About 67.12% of the organic Se existed in the soluble proteins.更多还原