【摘要】 β胡萝卜素极不稳定,在进行HPLC测定时常因标准品的失效而导致测量结果不准。文中对价格低廉、性质稳定的工业染料———苏丹Ⅰ代替β胡萝卜素标准品作为HPLC内标物进行了研究。得到相对校正因子为0 2 6 1,样品回收率97 98% ,方法精密度(RSD)为0 85 %。试验结果表明,苏丹Ⅰ作为内标物测定沙棘果渣中β胡萝卜素是一种简便、快速、准确且廉价的方法更多还原

【Abstract】 Unstable, invalidation of β-Carotene always caused an inaccurate result determined by HPLC. A simple、 rapid、accurate and cheap quantitative method of β-Carotene in marc of Sea buckthorn using Sudan Ⅰas an Internal Standard of HPLC was developed, Sudan Ⅰis a cheap and stable Industry dyestuff. The relative correction factor was 0.261. RSD of 0.85% (n=5). The average recovery of the method for β-Carotene was 97.98%(n=5).更多还原