【摘要】 首次研究了以辅酶Q10 (CoQ10 )主要侧链供给前体物质———茄尼醇和醌环供给前体———羟基苯甲酸和辅酶Q0 对粟酒裂殖酵母(Schizosaccharomycespromb 2 1794 - 2 3) 生产CoQ10 产量的影响,并对前体的转化工艺进行了初步研究。确定了适宜于粟酒裂殖酵母生长及高转化前体生成CoQ10 的条件为:酵母在2 8℃下,2 2 0r/min于发酵培养基中培养18h后,加入0 5 g/L茄尼醇继续发酵培养18h ,进行前体转化反应。结果表明,单独添加茄尼醇能达到最大产量33 1mg/L ,比对照样品增加了91% ,任2种前体物质共同添加都要比单独添加茄尼醇时产量低。茄尼醇和CoQ0 共同添加时,单位细胞胞外辅酶CoQ10 的产量达到最高的1 35mg/g ,比对照样品增加了117%。更多还原

【Abstract】 The effects of side-chain precursor-Solanesol, ubiquinone cicle precursors-phydroxybenzoic acid, and coenzyme Q₀ on biosynthesis of coenzyme Q₁₀ were studied. The feasibility of the precursors biotransformation to CoQ₁₀ by Schizosaccharomyces promb 2 1794-23 was also disscussed in the article S promb 2 1794-23 was cultrued under 28℃ and 220 r/min for 18 h, then 0 5g/L liquid solanesol was added and continue to ferment for 18 h for biotransformation The results showed that the precursors increased CoQ 10 production The largest CoQ 10 concentration, which was 90 1% higher than in control was obtained only with added solenesol When both Side-chain and ubiquinone circle precursors were added, CoQ 10 concentration became lower than that of when one of precursors was added. The coenzyme Q₁₀ in fermentation broth percell reached its maximum of 1 35 mg/g, and the yield increased 117% when both solanesol and coenzyme Q₀ were added.更多还原