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枸杞子水提物中多糖含量的测定
Detection of Polysaccharide in Lycium barbarum Extract
【摘要】 采用苯酚 硫酸法测定了枸杞子水提物中多糖的含量。测定波长 4 90nm ,多糖换算因子f =3 .2 6 ;在 7~ 4 9μg/mL范围内 ,其浓度与吸收度线性关系良好 ,R2 =0 .9984 ;多糖的平均回收率为 99 .74 % ,RSD为 1. 83%(n =6 ) ;测定枸杞子水提物中多糖的含量为 3 86 %。
【Abstract】 The content of polysaccharide in Lycium barbarum extract was measured by phenol-sulfuric acid method. Wavelength for measurement was 490 nm and converting factor was 3.26; good linearity ranged was obtained from 7~49 μg/mL, R2=0.9984; average recovery rate of was 99.74% with 1.83% of RSD(n=6). The extraltion rate was 3.86%. This is a simple, accurate and stable methoel. It can also be used for detecting of other polysaccharides.
【基金】 湖北省自然科学基金资助项目 (No .9552 5)
- 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2005年02期
- 【分类号】TQ464
- 【被引频次】72
- 【下载频次】1188