【摘要】 目的探讨深低温冻储在同种异体气管移植中的作用。方法选择近交系Lewis大鼠自体气管移植(L-L组),及F344和Lewis行未冻储(F-L组)、冻储(CF-L组)同种异体气管移植,分别在术后2、7、30天观察各组移植段光镜下免疫排斥反应变化。结果①CF-L组在移植术后各时间点与F-L组相比较,炎细胞浸润程度及上皮下组织增殖相比显著降低(P<0.05)。②CF-L组PBMC内γ-IFN表达量在术后2、7、30天均明显低于F-L组(P<0.01)。③F-L组在移植术后2、7、30天,凋亡细胞计数均较L-L组和CF-L组高(P>0.05)。结论深低温冻储可能通过调节同种异体气管移植术后Th型细胞因子的变化和移植物细胞凋亡,从而抑制了免疫排斥反应。更多还原

【Abstract】 Objective To investigate the effect of cryopreservation on tracheal allotransplantation. Methods Inbred Lewis donor(L-L group) and fresh or cryopreserved(1 month) F344 donor(F-L or CF-L group) tracheal segments were orthotopically transplanted in Lewis rat. Each groups were observed and compared. Results (1)On each examination day after transplantation, the intensity of inflammatory cells and thickness of subepithelium in CF-L group were significantly lower than those in the F-L group (P<0.05). (2)On day 2, 7, 30, the γ-IFN expression of PBMC in CF-L group was always lower than that in F-L group on each examination day (P<0.01). (3)On day 2, 7, 30, much more apoptotic cells were observed in sections of grafts of F-L group and the number of cells was higher than that in L-L and CF-L group(P<0.05). Conclusion The cryopreservation may produce immunosuppressive results because it modulate apoptosis of tracheal allografts and Th cytokine of PBMC.更多还原